The effect of IBMX and prolactin on functional status of cryopreserved bull spermatozoa

Purpose: investigate the effect of IBMX (activator protein phosphorylation) and prolactin (PRL) on functional state cryopreserved bovine spermatozoa using inhibitory analysis. Materials methods. Frozen-thawed semen samples from 60 black-and-white bulls was used in experiments. For capacitation, cells were incubated Sp-TALP medium supplemented with 6 mg/ml serum albumin various compounds: an inductor capacitation (IBMX at concentrations 1 μM, 10 50 100 μM), hormone (PRL ng, ng) inhibitors kinases C (Ro 31-8220 a concentration ng/ml) kinase A (H-89 μM). The incubation carried out 38°C atmosphere 5% CO 2 , 98% humidity for 4 hours. status determined by chlortetracycline test. Results. It shown that all experimental did not affect post-ejaculatory maturation (capacitation acrosome reaction) spermatozoa, while PRL (1-100 promoted reaction capacitated cells. In presence inhibitor, there decrease number increase acrosome-reactive under action μM no changes inhibitor. Also, case inhibition PRL-related stimulation canceled, usage H-89 mediated PRL. Thus, influence processes thawed studied Conclusion. At capacital stage, ratio deconved status. Prode also contributed to passage acrosomous rolled after defrosting. Inhibition when incubating has acrosomal exocytosis ripped this process PRR, inhibitor changed direction increasing percentage rate occasion I participate intracellular provided